Acrolein, a neurotoxin found in tobacco smoke that is thought to increase pain in people with spinal cord injury has now been shown to accumulate in mice exposed to the equivalent of 12 cigarettes daily over a short time period.
If acrolein is exacerbating pain, its concentration in the body could be reduced using the drug hydralazine, which has been approved by the US Food and Drug Administration for hypertension, according to Riyi Shi, a professor in Purdue University’s Department of Basic Medical Sciences, College of Veterinary Medicine, and Weldon School of Biomedical Engineering. The drug has been shown to be effective in reducing acrolein levels in research animals, and Shi is working to develop a low-dose version for that purpose in humans.
Acrolein is produced within the body after nerve cells are damaged. In spinal cord injury and in multiple sclerosis, the myelin insulation surrounding nerve cells is destroyed and the nerve fibres themselves are damaged by acrolein. The toxin acrolein is also found in air pollutants including tobacco smoke and vehicle exhaust.
Mice were exposed to a level of acrolein equivalent to 12 cigarettes per day over three weeks. Previous research has focused on acrolein accumulation in the respiratory system but not in the bloodstream and spinal cord. It is known that acrolein is accumulated in urine in human smokers after years of smoking.
“This is the first animal study demonstrating that an acute short term of weeks of smoking could also cause acrolein to accumulate in urine and more importantly in spinal cord tissue, a part of central nervous system known to be vulnerable to acrolein,” says Shi.
The researchers documented the concentration of biochemical markers for acrolein in the urine and spinal cord. Findings, published in the Neuroscience Bulletin, indicate the accumulation of the toxin was about 50% higher than normal, a level known to have pathological implications.
“The data indicated that acrolein is absorbed into the circulatory system and some enters the nervous system,” Shi says. “It is expected that these findings may facilitate further studies to probe the pathological role of acrolein in the nervous system resulting from smoke and other external sources through long and short term, both active and passive exposure.”
“It is already known that smoking can increase pain for people with spinal cord injury and worsen the condition of multiple sclerosis, but we do not know exactly why,” Shi explains. “I am saying that acrolein might be the key culprit here and that inhaled acrolein could intensify multiple sclerosis and increase pain sensation.”